Office: Magee Women's Research Institute
Dr. Rohan is a Professor in the Department of Pharmaceutical Sciences in the School of Pharmacy. She also holds appointments in the Department of Obstetrics, Gynecology, and Reproductive Sciences in the School of Medicine and the Clinical Translational Science Institute at the Univerisity of Pittsburgh. Additionally, she is an Associate Investigator at the Magee Womens Research Institute. She obtained a Bachelor of Science in Chemical Engineering from West Virginia University and a Ph.D. in Pharmaceutics from the University of Pittsburgh School of Pharmacy. After completion of her Ph.D. work Dr. Rohan completed a Postdoctoral Fellowship in the Department of Obstetrics, Gynecology, and Reproductive Sciences in the area of Mucosal Immunology at the University of Pittsburgh.
The basis of Dr. Rohan’s research lies in vaginal and cervical delivery as applied in the areas of infectious disease and gynecologic oncology. Particular interests are in studying the role of chemical, physical, and biological properties of vaginal and cervical tissues and fluids in the development of vaginal and cervical products. Specifically, this knowledge is being utilized to develop a product for prophylactic use against Sexually Transmitted Diseases and HIV. Her research also involves the development and optimization of imaging techniques for sentinel node identification in cervical cancer as well as novel delivery systems for chemotherapeutic agents.
Design and development of drug delivery systems for STI/HIV prevention and gynecologic oncology applications.
1. Gupta, P., Lackman-Smith, C., Ratner, D., Rohan, L. C.,Patton, D., Bharat Ramratnam, B and Alexander M Cole. Antiviral Activity of Retrocycline RC-101, a Candidate Microbicide against Cell-Associated HIV-1. AIDS Research and Human Retroviruses 2012 Aug 27. PMID:22924614
2. Huang, Y, Lu, J, Gao, X, Li, J, Sun, M, Stolz, D, Venkataramanan R, Rohan L, Li S, PEG-Derivatized Embelins as a Dual Function Carrier for the Delivery of Poorly Water-Soluble Anti-Cancer Drugs. Bioconjug Chem. 2012 Jun 20. [Epub ahead of print]PMID:22681537
3. Gupta,P, Ratner,D., Ding,M., Patterson,B., Rohan,LC., Reinhart, TA., Ayyavoo,V, Huang, X., Patton, DL, Ramratnam,B and Cole, AM. Retrocyclin RC-101 blocks HIV-1 transmission across cervical mucosa in an Organ Culture. J Acquir Immune Defic Syndr. 2012 May 15 PMID: 22611520
4. Dezzutti,CS, Rohan, LC, Wang, L, Uranker K, Shelter C, Cost M, Lynam, JD, Friend, D. Reformulated Tenofovir Gel for use as a Dual Compartment Microbicide. J Antimicrob Chemother. 2012 May 11. [Epub ahead of print] PMID:22581908
5. Cost, MR, Dezzutti, CS, Clark, M, Akil, A Rohan, LC. Characterization of UC781/Tenofovir Combination Gel Products for HIV-1 Prevention. Antimicrob Agents Chemother. 2012 Jun;56(6):3058-66. Epub 2012 Mar 19. PMID:22430977
6. Ham A S., Rohan, L C., Boczar, A., Yang, L, Buckheit, KW, Buckheit, RW. Vaginal Film Drug Delivery of the Pyrimidinedione IQP-0528 for the Prevention of HIV Infection. Pharm Rsh. 2012 Mar 6. [Epub ahead of print] PMID:22392331
7. Moncla BJ, Pryke K, Rohan LC, Yang H. Testing of viscous anti-HIV microbicides using Lactobacillus. J Microbiol Methods. 2012 Feb;88(2):292-6. Epub 2011 Dec 24.PubMed PMID: 22226641; PubMed Central PMCID: PMC3271802.
8. Rinehart MT, Drake TK, Robles FE, Rohan LC, Katz D, Wax A. Time-resolved imaging refractometry of microbicidal films using quantitative phase microscopy. J Biomed Opt. 2011 Dec;16(12):120510. PubMed PMID: 22191912; PubMed CentralPMCID: PMC3253752.
9. Wang L, Sassi AB, Patton D, Isaacs C, Moncla BJ, Gupta P, Rohan LC. Development of a liposome microbicide formulation for vaginal delivery of octylglycerol for HIV prevention. Drug Dev Ind Pharm. 2011 Dec [Epub ahead ofprint] PubMed PMID: 22149387.
10. Moncla, B. J.,Pryke, K., Rohan, L. C. Graebing, P. W. Degradation of Naturally occurring and engineered antimicrobial peptides by proteases. Advances in Bioscience and Biotechnology. 2011 Dec; 2(6). 404-408
11. Li M, Patton DL, Cosgrove-Sweeney Y, Ratner D, Rohan LC, Cole AM, Tarwater PM, Gupta P, Ramratnam B. Incorporation of the HIV-1 microbicide cyanovirin-N in a food product.. J Acquir Immune Defic Syndr. 2011 Sep 15. PMID:21926631
12. Mahalingam A, Jay JI, Langheinrich K, Shukair S, McRaven MD, Rohan LC, Herold BC, Hope TJ, Kiser PF.
Inhibition of the transport of HIV in vitro using a pH-responsive synthetic mucin-like polymer system. Biomaterials. 2011 Nov; 32(33):8343-55. Epub 2011 Aug 27. PMID: 21875751
13. Yang H, Parniak MA, Dezzutti C, Hillier SL, and Rohan, LC. A Thermodynamic Study of the Cyclodextrin-UC781 Inclusion Complex using HPLC Methods. Journal of Inclusion Phenomena and Macrocyclic Chemistry (30 July 2011), pp. 1-7.
14. Isaacs, C, Xu,W, Merz, G, Hillier, SL, Rohan, LC, Wen, G. Digallate Dimers of (-)-Epigallocatechin Gallate Inactivate Herpes Simplex Virus. Antimicrobial Agents and Chemotherapy. 2011 Sep 26. PMID:21947401
15. Sassi AB, Bunge KE, Hood BL, Conrads TP, Cole AM, Gupta P, Rohan LC. Preformulation and stability in biological fluids of the retrocyclin RC-101, a potential anti-HIV topical microbicide.. AIDS Res Ther. 2011 Jul 29; 8:27. PMID:21801426
16. Akil A, Parniak M, Dezzutti C, Moncla B, Cost M, Li M, Rohan LC. Development and Characterization of a Vaginal Film Containing Dapivirine, a Non-nucleoside Reverse Transcriptase Inhibitor (NNRTI), for prevention of HIV-1 Sexual Transmission. Drug Delivery and Translational Research. 2011 1:209-222.
17. Wang L, Schnaare RL, Dezzutti C, Anton P, Rohan, LC. Rectal Microbicides: Clinically relevant approach to design of rectal specific placebo formulations. AIDS Research and Therapy. 2011 March 7:8-12. PMID 21385339
18. Sassi, A.B., M.R. Cost, A.L. Cole, A. M. Cole, D.L. Patton, P. Gupta, Rohan, LC. Formulation Development of Retrocyclin-1 (RC-101) as an anti-HIV vaginal microbicide. Antimicrobial Agents and Chemotherapy. 2011 May 55(5): 2282-9. PMID 21321138
19. Mahalingam A, Simmons A, Ugaonkar S, Watson K, Dezzutti C, Rohan LC, Buckheit R., Kiser P. Vaginal microbicide gel for the delivery of IQP-0528, a pyrimidinedione analog with a dual mechanism of action against HIV-1. Antimicrob Agents Chemother. 2011 April 55(4): 1650-60. PMID 21245437
20. Garg S, Goldman D, Krumme M, Rohan LC, Smoot S, Friend D. Advances in development, scale up and manufacturing of microbicide gels, films, and tablets. Antiviral Research. Volume 88 Supplement 1 (December 2010) S19-S29. PMID:21109064
21. Cole AM, Patton DL, Rohan LC, Cole AL, Cosgrove-Sweeney Y, Rogers NA, Ratner D, Sassi AB, Lackman-Smith C, Tarwater P, Ramratnam B, Ruchala P, Lehrer RI, Waring AJ, Gupta P. The formulated microbicide RC-101 was safe and antivirally active following intravaginal application in pigtailed macaques. PLoS One. 2010 Nov 29; 5(11):e15111. PMID:21124745
22. Rohan, LC, Moncla BJ, Kunjara, RP, Cost M, Huang Y, Gai, F, Billotto,N, Lyman, JD, Pryke, K, Graebing P, Hopkins N, Rooney J, Friend, D. and Dezzutti CS,. In vitro and Ex vivo Testing of Tenofovir shows it is Effective as an HIV-1 Microbicide. PloS ONE Volume 5, Issue 2 (February 2010), e9310
23. Ham, A. S., M. Cost, P. Graebing, D. L. Patton, M. Lederman, Rohan L.C. Targeted Delivery of PSC-RANTES for HIV-1 Prevention using Biodegradable Nanoparticles. Pharmaceutical Research. Volume 26, Issue3 (2009), Page 502.
24. Yang, H, M. A. Parniak, C. E. Isaacs, S. L. Hillier, L. C. Rohan. Characterization of Cyclodextrin inclusion complexes of the Anti-HIV Non-Nucleoside Reverse Transcriptase Inhibitor UC781. The AAPS Journal. Volume 10. Issue 4 (2008). Page 606.
25. Mu, Y, Klamerus, M.,Miller, T, Rohan, LC., Graham, SH, Poloyac, SM. Intravenous Formulation of HET0016 for Selective Inhibition of Rat Brain 20-Hydroxyeicosatetraenoic Acid (20-HETE) Formation. Drug Metabolism and Disposition. 36(11): 2324-30 (2008 Nov)
26. Fader A. N., R. P. Edwards, M. Cost, A. Kanbour-Shakir, J. Schwartz, J. Kelley, P. Comerci, J. Sukumvanich, R. P. Sumkin, L. C. Rohan. Sentinel Lymph Node Biopsy in Early Stage Cervical Cancer: Utility of Intraoperative versus Postoperative Assessment. Gynecologic Oncology. 111(1):13-7 (2008 Oct)
27. Sassi, A, C. Isaacs, B. Moncla, P. Gupta, S. L. Hillier, L. C. Rohan. Effects of physiological fluids on physical chemical characteristics and activity of topical vaginal microbicide products. Journal of Pharmaceutical Sciences. 97 (8):3123-39 (2008 Aug)
28. Isaacs, C. E., W. Xu, J. H. Jia, L. C. Rohan, G. Wen, S. L. Hillier. Epigallocatechin Gallate Inactivates Clinical Isolates of Herpes Simplex Virus. Antimicrobial Agents and Chemotherapy. 52(3):962-970 (2008)
29. Patton, D.L., S. Y. T. Cosgrove, J. E. Balkus, L. C. Rohan, B. J. Moncla, M. A. Parniak, S. L. Hillier. Preclinical Safety Assessments of UC781 Anti-HIV Topical Microbicide Formulations. Antimicrobial Agents and Chemotherapy. 51: 1608-1615. (2007)
30. Gupta, P, D. Dampf, B. K. Patterson, L. Rohan, M. Parniak, C. E. Isaacs, S. L. Hillier. Use of Frozen-thawed Cervical Tissues in the Organ Culture System to Measure anti-HIV activities of candidate microbicides. AIDS Research & Human Retroviruses. 22(5):419-24, (2006 May)
Films as a Vaginal Delivery Drug Platform
Release Assays from EVA Ring Segments
U01 AI068633 / Microbicide Trials Network
(Rohan: Central Core Laboratory Co Director: Rohan, LC)
U19 AI082639/ Alternative Formulations of Tenofovir and UC781
(Rohan: CoPI for Program and PI for Project 1 and Core C)
U19 AI082637 / Combination HIV Antiretroviral Rectal Microbicide Program
(Rohan: Core Director)
RFA-AI-09-057 / RT Inhibitor CSIC and RC101 as Microbicides
(Rohan: Core Director)
RFA-AI-09-021 / Development of a Novel Nanoparticle Pyrimidinedione Vaginal Polymeric Film as an Anti-HIV Microbicide
Microbicide Properties of RT Inhibitor Combinations
Aminoglycoside Microbicides Restore Natural Expression of Anti-HIV Retrocyclins
Exploring HIV-Host Interactions in the Female Reproductive Tract
Development of Vaginal Quick Dissolve Films Containing Combinations of Maraviroc/Tenofovir and Dapivirine/Tenofovir
International Partnership for Microbicides
Development of Natural Products for HSV Prevention