Directory Profile

Wen Xie, MD, PhD

Office: 306 Salk Pavilion, 335 Sutherland Drive, Pittsburgh PA 15261

Phone: 412-648-9941

Fax: 412-648-1664

Email: wex6@pitt.edu

Wen Xie (MD, PhD) is Chairman of the Department of Pharmaceutical Sciences, and Joseph Koslow Endowed Professor in Pharmaceutical Sciences at the School of Pharmacy, and Professor of Pharmacology & Chemical Biology at the School of Medicine.

Dr. Xie obtained his MD degree from Peking University Health Science Center, and PhD in Cell Biology from University of Alabama at Birmingham. He completed a postdoctoral fellowship with Dr. Ronald M. Evans at the Salk Institute in La Jolla, California before joining the University of Pittsburgh.

The research focus of Dr. Xie's laboratory is nuclear receptor-mediated transcriptional regulation of genes that encode drug metabolizing enzymes and drug transporters. In addition to metabolizing drugs, the same enzyme and transporter systems are responsible for the homeostasis of endogenous chemicals. Therefore, besides drug metabolism and disposition, this regulation has broad implications in many human diseases, including liver diseases (fatty liver, liver fibrosis, liver cancer, and autoimmune hepatitis), endocrine disorders, metabolic syndrome, and cancers. Dr. Xie’s research is conducted using a combination of molecular biology and genetically engineered mice that include tissue and cell type-specific transgenic, knockout and humanized mice.

Dr. Xie is the author or coauthor of more than 200 journal articles and book chapters and he has delivered over 200 invited lectures at conferences and universities. Dr. Xie’s papers have been cited for more than 12,000 times and his H-index is 60 as of January 2021. Dr. Xie has edited and published two books: "Nuclear Receptors in Drug Metabolism" (Wiley 2008) and "Drug Metabolism in Diseases" (Elsevier 2016). Dr. Xie has served as ad hoc reviewer for NIH and DOD Study Sections and for over 100 scientific journals.

Among his achievements, Dr. Xie is the recipient of the University of Pittsburgh Chancellor’s Distinguished Research Award, Awards from International Society for the Study of Xenobiotics (ISSX) and American Society for Pharmacology and Experimental Therapeutics (ASPET) Division for Drug Metabolism and Disposition, the Joseph Koslow Endowed Chair in Pharmaceutical Sciences, University of Pittsburgh School of Pharmacy Graduate Program Faculty Member of the Year Award, and National Institute of Environmental Health Sciences (NIEHS) Revolutionizing Innovative, Visionary Environmental health Research (RIVER) R35 Award.

Dr. Xie's lab is currently having openings for MS and PhD students, postdoctoral fellows, and visiting students/scholars. Dr. Xie is passionate about training next generations of scientists and many of his trainees went on having their successful careers in academia and industry. If interested, please email Dr. Xie at wex6@pitt.edu

Nuclear receptor-mediated transcriptional regulation of genes that encode drug metabolizing enzymes and drug transporters, and implications of this regulation in xenobiotic and endobiotic homeostasis and human diseases.

Dr. Xie's lab is currently having openings for MS and PhD students, postdoctoral fellows, and visiting students/scholars. Dr. Xie is passionate about training next generations of scientists and many of his trainees went on having their successful careers in academia and industry. If interested, please email Dr. Xie at wex6@pitt.edu

Lu P, Yan J, Liu K, Garbacz WG, Wang P, Xu M, Ma X, Xie W. Activation of Aryl Hydrocarbon Receptor Dissociates Fatty Liver from Insulin Resistance by Inducing FGF21. Hepatology 61: 1908-1919 (2015)

Guo Y, Hu B, Huang H, Tsung A, Gaikwad N, Xu M, Jiang M, Ren S, Fan J, Billiar TR, Huang M, Xie W. Estrogen sulfotransferase is an oxidative stress responsive gene that gender-specifically affects liver ischemia/reperfusion injury. J Biol Chem. 290: 14754-14764 (2015)

Hu B, Guo Y, Garbacz WG, Jiang M, Xu M, Huang H, Tsung A, Billiar TR, Ramakrishnan SK, Shah YM, Lam KSL, Huang M, Xie W. Fatty acid binding protein-4 (FABP4) is a hypoxia inducible gene that sensitizes mice to liver ischemia/re-perfusion injury. J Hepatol 63: 855-862 (2015)

Chai X, Guo Y, Jiang M, Hu B, Li Z, Fan J, Deng M, Billiar TR, Kucera H, Gaikwad NW, Xu M, Lu P, Yan J, Fu H, Liu Y, Yu L, Huang M, Zeng S, Xie W. Estrogen sulfotransferase ablation sensitizes mice to sepsis. Nat Commun. 6: 7979 (2015)

Jiang M, Klein M, Zanger UM, Mohammad MK, Cave MC, Gaikwad NW, Dias NJ, Selcer KW, Guo Y, He J, Zhang X, Shen Q, Qin W, Li J, Li S, Xie W. Inflammatory regulation of steroid sulfatase, a novel mechanism to control estrogen homeostasis and inflammation in chronic liver disease. J Hepatol. 2016; 64: 44-52

Gao J, Yan J, Xu M, Ren S, Xie W. CAR suppresses hepatic gluconeogenesis by facilitating the ubiquitination and degradation of PGC1α. Mol Endocrinol 29: 1558-1570 (2015)

Zhao Z, Xu D, Li S, He B, Huang Y, Xu M, Ren S, Li S, Wang H, Xie W. Activation of liver X receptor attenuates oleic acid-induced acute respiratory distress syndrome. Am J Pathol. 186: 2614-2622 (2016)

Garbacz WG, Lu P, Miller TM, Poloyac SM, Eyre NS, Mayrhofer G, Xu M, Ren S, Xie W. Hepatic overexpression of CD36 improves glycogen homeostasis and attenuates high-fat diet induced hepatic steatosis and insulin resistance. Mol Cell Biol. 36: 2715-2727 (2016)

Chen Y, Xia R, Huang Y, Zhao W, Li J, Zhang X, Wang P, Venkataramanan R, Fan J, Xie W, Ma X, Lu B, Li S. An immunostimulatory dual-functional nanocarrier that improves cancer immunochemotherapy. Nat Commun 7: 13443 (2016).

Niu Y, Xu M, Slagle BL, Huang H, Li S, Guo GL, Shi G, Qin W, Xie W. Farnesoid X receptor (FXR) ablation sensitizes mice to hepatitis B virus X protein (HBx)-induced hepatocarcinogenesis. Hepatology 65: 893-906 (2017)

Hu B, Li Y, Gao L, Guo Y, Zhang Y, Chai X, Xu M, Yan J, Lu P, Ren S, Zeng S, Liu Y, Xie W, Huang M. Hepatic induction of fatty acid binding protein 4 (FABP4) plays a pathogenic role in sepsis in mice. Am J Pathol 187: 1059-1067 (2017)

Zheng Z, Zhao Z, Li S, Lu X, Jiang M, Lin J, An Y, Xie Y, Xu M, Shen W, Guo GL, Huang Y, Li S, Zhang X, Xie W. Altenusin, a non-steroidal microbial metabolite, attenuates non-alcoholic fatty liver disease by activating the farnesoid X receptor. Mol Pharmacol 92: 425-436 (2017)

Garbacz WG, Jiang M, Xu M, Yamauchi J, Dong HH, Xie W. Sex- and tissue-specific role of estrogen sulfotransferase in energy homeostasis and insulin sensitivity. Endocrinology 158: 4093-4104 (2017)

Choi Y, Zhou D, Barbosa AC, Niu Y, Guan X, Xu M, Ren S, Nolin TD, Liu Y, Xie W. Activation of constitutive androstane receptor ameliorates renal ischemia-reperfusion induced kidney and liver injury. Mol Pharmacol 93: 239-250 (2018)

Kim YC, Seok S, Byun S, Kong B, Zhang Y, Guo G, Xie W, Ma J, Kemper B, Kemper J. AhR and Shp regulate phosphatidylcholine and S-adenosylmethionine levels in the one-carbon cycle. Nat Commun 9: 540 (2018)

Bi Y, Shi X, Zhu J, Guan X, Garbacz WG, Huang Y, Gao L, Yan J, Xu M, Ren S, Ren S, Liu Y, Ma X, Li S, Xie W. Regulation of cholesterol sulfotransferase SULT2B1b by HNF4a constitutes a negative feedback control of hepatic gluconeogenesis. Mol Cell Biol 38: e00654-17 (2018)

Wang Z, Yang B, Zhang M, Guo W, Wu Z, Wang Y, Jia L, Li S; Cancer Genome Atlas Research Network, Xie W, Yang D. lncRNA Epigenetic Landscape Analysis Identifies EPIC1 as an Oncogenic lncRNA that Interacts with MYC and Promotes Cell-Cycle Progression in Cancer. Cancer Cell 33: 706-720 (2018)

Evers R, Piquette-Miller M, Polli J, Russel F, Sprowl J, Tohyama K, Ware J, de Wildt S, Xie W, Brouwer K. Disease-associated changes in drug transporters may impact the PK and/or toxicity of drugs: a white paper from the International Transporter Consortium. Clin Pharmacol Ther 104: 900-915 (2018)

Garbacz WG, Uppal H, Yan J, Xu M, Ren S, Stolz DB, Huang M, Xie W. Chronic activation of LXR sensitizes mice to high cholesterol diet induced gut toxicity. Mol Pharmacol 94: 1145-1154 (2018)

Bi Y, Jiang M, Guo W, Guan X, Xu M, Ren S, Yang D, Gaikwad NW, Selcer KW, Xie W. Sex-dimorphic and sex hormone-dependent role of steroid sulfatase in adipose inflammation and energy homeostasis. Endocrinology 159: 3365-3377 (2018)

Lu P, Cai X, Guo Y, Xu M, Tian J, Locker J, Xie W. Constitutive activation of the human aryl hydrocarbon receptor in mice promotes hepatocarcinogenesis independent of its coactivator Gadd45b. Toxicol Sci 167: 581-592 (2019)

Guo GL, Xie W. Metformin action through the microbiome and bile acids. Nat Med. 24: 1789-1790 (2018)

Chen L, Liu Q, Tang Q, Kuang J, Li H, Pu S, Wu T, Yang X, Li R, Zhang J, Zhang Z, Huang Y, Li Y, Zou M, Jiang W, Li T, Gong M, Zhang L, Wang H, Qu A, Xie W, He J. Hepatocyte-specific Sirt6 deficiency impairs ketogenesis. J Biol Chem 294: 1579-1589 (2019)

An Y, Wang P, Xu P, Tung HC, Xie Y, Kirisci L, Xu M, Ren S, Tian X, Ma X, Xie W. An unexpected role of cholesterol sulfotransferase and its regulation in sensitizing mice to acetaminophen induced liver injury. Mol Pharmacol 95: 597-605 (2019)

Xie Y, Xu M, Deng M, Li Z, Wang P, Ren S, Guo Y, Ma X, Fan J, Billiar TR, Xie W. Activation of pregnane X receptor sensitizes mice to hemorrhagic shock induced liver injury. Hepatology 70: 995-1010 (2019)

Shehu A, Lu L, Wang P, Zhu J, Wang Y, Yang D, McMahon D, Xie W, Gonzalez FJ, Ma X. Pregnane X receptor activation potentiates ritonavir hepatotoxicity. J Clin Invest 129: 2998-2903 (2019)

Yan J, Tung HC, Li S, Niu Y, Garbacz WG, Lu P, Bi Y, Li Y, He J, Xu M, Ren S, Monga SP, Schwabe RF, Yang D, Xie W. Aryl hydrocarbon receptor signaling prevents activation of hepatic stellate cells and liver fibrogenesis in mice. Gastroenterology 157: 793-806 (2019)

Feng Y, Xie Y, Xu M, Li L, Selcer KW, Oberly PJ, Poloyac SM, Wang H, Li C, Dong F, Yu C, Xie W. Hepatic steroid sulfatase critically determines estrogenic activities of conjugated equine estrogens in human cells in vitro and in mice. J Biol Chem. 294: 12112-12121 (2019)

Wang P, Sachar M, Lu J, Shehu AI, Zhu J, Chen J, Liu K, Anderson KE, Xie W, Gonzalez F J, Klaassen CD, Ma X. The essential role of the transporter ABCG2 in the pathophysiology of erythropoietic protoporphyria. Sci Adv 5: eaaw6127 (2019)

Xie Y, Barbosa AC, Xu M, Oberly PJ, Ren S, Gibbs RB, Poloyac SM, Song W, Fan J, Xie W. Hepatic estrogen sulfotransferase distantly sensitizes mice to hemorrhagic shock-induced acute lung injury. Endocrinology 161: pii: bqz031 (2019)

Barbosa ACS, Zhou D, Xie Y, Choi YJ, Tung HC, Chen X, Xu M, Gibbs RB, Poloyac SM, Liu S, Yu Y, Luo J, Liu Y, Xie W. Inhibition of estrogen sulfotransferase (SULT1E1/EST) ameliorates ischemic acute kidney injury in mice. J Am Soc Nephrology 31: 1496-1508 (2020)

Zhang J, Li Y, Liu Q, Huang Y, Li R, Wu T, Zhang Z, Zhou J, Huang H, Tang Q, Huang C, Zhao Y, Zhang G, Jiang W, Mo L, Zhang J, Xie W, He J. Sirt6 alleviated liver fibrosis by deacetylating conserved lysine 54 on Smad2 in hepatic stellate cells. Hepatology 2020 June 14. Online ahead of print.

Gao L, Li B, Wang J, Shen D, Yang M, Sun R, Tung TC, Xu M, Ren S, Zhang M, Yang D, Lu B, Wang H, Liu Y, Xie W. Activation of liver X receptor α sensitizes mice to T-cell mediated hepatitis. Hepatol Commun 4: 1664-1679 (2020)

Fang Z, Wang Y, Wang Z, Xu M, Ren S, Yang D, Hong M, Xie W. ERINA is an estrogen-responsive lncRNA that drives breast cancer through the E2F1/RB1 pathway. Cancer Res 80: 4399-4413 (2020) PMID: 32826278

Guo W, Wang Y, Yang M, Wang Z, Wang Y, Chaurasia S, Wu Z, Zhang M, Yadav GS, Rathod S, Concha-Benavente F, Fernandez C, Li S, Xie W, Ferris RL, Kammula US, Lu B, Yang D. LincRNA-immunity landscape analysis identifies EPIC1 as a regulator of tumor immune evasion and immunotherapy resistance. Sci Adv 7: eabb3555 (2021) PMID: 33568470

Xu P, Xi Y, Zhu J, Zhang M, Luka Z, Stolz DB, Cai X, Xie Y*, Xu M, Ren S, Huang Z, Yang D, York JD, Ma X, Xie W. Intestinal sulfation is essential to protect against colitis and colonic carcinogenesis. Gastroenterology 161: 271-286 (2021) PMID: 33819483

Xi Y, Li Y, Xu P, Li S, Liu Z, Tung HC, Cai X, Wang J, Huang H, Wang M, Xu M, Ren S, Li S, Zhang M, Lee YJ, Huang L, Yang D, He J, Huang Z, Xie W. The anti-fibrotic drug pirfenidone inhibits liver fibrosis by targeting the small oxidoreductase glutaredoxin-1. Sci Adv (Accepted)

University of Pittsburgh Chancellor’s Distinguished Research Award

The James R. Gillette International Society for the Study of Xenobiotics (ISSX) North American New Investigator Award

The American Society for Pharmacology and Experimental Therapeutics (ASPET) Division for Drug Metabolism Early Career Achievement Award

The Joseph Koslow Endowed Chair in Pharmaceutical Sciences

University of Pittsburgh School of Pharmacy Graduate Program Faculty Member of the Year Award

National Institute of Environmental Health Sciences (NIEHS) Revolutionizing Innovative, Visionary Environmental health Research (RIVER) R35 Award

Department of Biological Sciences, Duquesne University, Pittsburgh, PA. February 6, 2015.

Experimental Biology 2014 Meeting/ASPET, Boston, MA. March 28-April 1, 2015.

Department of Pharmacology, School of Pharmacy, Second Military Medical University, Shanghai, China. April 10, 2015.

1st Sunrise Symposium for Drug Metabolism, Pharmacokinetics and Dynamics, Nanjing, China. April 11-13, 2015.

Annual Meeting of the Canadian Society of Pharmaceutical Sciences (CSPS), Toronto, Ontario, May 26-29, 2015

School of Pharmaceutical Sciences, Anhui Medical University. Hefei, China. August 19, 2015.

Department of Pharmacology and Toxicology, Michigan State University, East Lansing, Michigan. October 28, 2015.

UC Davis Cancer Center, Sacramento, CA. November 12, 2015.

Department of Cancer Biology, the Lerner Research Institute of the Cleveland Clinic. Cleveland, Ohio. November 24, 2015.

2nd Ling-Nan International DMPK Summit Conference. Guangzhou, China. December 4-6, 2015.

Department of Pharmacology, College of Pharmacy and Pharmaceutical Sciences, University of Toledo. Toledo, Ohio. March 23, 2016.

School of Pharmaceutical Sciences, Peking University Health Science Center. Beijing, China. June 3, 2016.

Department of Biology, South University of Science and Technology of China. Shenzhen, China. June 6, 2016.

11th Biennial Meeting of Chinese Biological Investigators Society (CBIS), Chengdu, China. July 29-August 2, 2016.

Center for Molecular Toxicology and Carcinogenesis, Penn State University, University Park, PA. September 14, 2016

21th Microsomes and Drug Oxidations (MDO) Meeting (Speaker and Session Chair). Davis, CA. October 2-6, 2016.

2016 American Association of Pharmaceutical Scientists (AAPS) Annual Meeting. Denver, Colorado. November 13-17, 2016.

Guangdong Provincial Key Laboratory of Liver Disease, the Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, China. December 8, 2016.

School of Pharmaceutical Sciences, Sun Yat-Sen University. Guangzhou, China. December 8, 2016.

2st Sunrise Symposium for Drug Metabolism, Pharmacokinetics and Dynamics, Xiamen, China. December 9-11, 2016.

School of Basic Medical Sciences, Capital Medical University, Beijing, China. December 14, 2016.

Department of Departments of Biopharmaceutical Sciences, University of Illinois at Chicago College of Pharmacy, Chicago, IL. March 7, 2017.

Gordon Research Conference on Drug Metabolism. Holderness School, Plymouth, New Hampshire. July 9-14. 2017

Department of Hepatology and Gastroenterology, the First Affiliated Hospital of Norman Bethune College of Medicine, Jilin University, Jilin, China. August 1, 2017.

Shenyang DMPK Workshop, Shenyang Pharmaceutical University, Shenyang, Liaoning, China. August 2-4, 2017.

Gordon Research Conference on Cellular & Molecular Mechanisms of Toxicology (Speaker and Session Chair). Proctor Academy, Andover, NH, August 13-18, 2017.

2017 American Association for The Study of Liver Diseases (AASLD) Meeting, Washington DC. October 20-24, 2017

3nd Ling-Nan International DMPK Summit Conference. Guangzhou, China. December 1-3, 2017.

8th Xiamen Winter Symposium on Nuclear Receptors. Xiamen, China. December 8-11, 2017.

Department of Physiology and Pathophysiology, Peking University Health Science Center. Beijing, China. March 23, 2018.

6th Asia Pacific International Society for the Study of Xenobiotics (APISSX Meeting), Hangzhou, China. May 11-14, 2018.

22nd Microsomes and Drug Oxidations (MDO) Meeting. Kanazawa, Japan. October 1-5, 2018.

Joy Goodwin Lectureship Seminar, Auburn University College of Veterinary Medicine. Auburn, Alabama. October 31, 2018

11th Immune-Mediated Digestive Diseases Forum & Intestinal Mucosal Barrier and Related Diseases Forum. Beijing, China. June 1-2, 2019.

Salk Symposium on Nuclear Receptors, The Salk Institute for Biological Sciences, La Jolla, CA. November 7-8, 2019

Liver research seminar in honor of Dr. Hutterer. Northeast Ohio Medical University (NEOMED), Rootstown, Ohio. November 10, 2020

Department of Pharmacology and Toxicology, University of Arizona College of Pharmacy. Tuson, Arizona. November 17, 2020.

Environmental Toxicology Seminar Series. University of California at Riverside, Riverside, California. January 27, 2021

ASPET/EB2021, Online Conference, April 27-30, 2021.

FASEB Conference on The Steroid Hormones and Receptors in Health and Disease. Online Conference, May 25-27, 2021.

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